《校花被出租车司机强H》Ep. 186在线观看 - 英语HD...《校花被当公共精厕》校花被当公共精厕免费阅读全文无弹窗...
本次发行采用向参与战略配售的投资者定向配售、网下向符合条件的投资者询价配售及网上向持有深圳市场非限售A股股份和非限售存托凭证市值的社会公众投资者定价发行相结合的方式进行。
2025年01月01日,直到有一天,她碰到了一个跟她兴趣相投、想法也差不多的男生,说起来,她对摇滚乐的那份热爱,还是受了这男生的影响呢。
《校花被出租车司机强H》Ep. 186在线观看 - 英语HD...《校花被当公共精厕》校花被当公共精厕免费阅读全文无弹窗...
对于夏天与川西
2024-07-15 19:34·曲靖珠江网第三条是需外出乞食。无论和尚还是比丘尼,乞食都是最基本的修行手段。她们每隔一段时间会出门去乞食,到普通人家里或者向路人乞问一些食物。大家乞到的食物最后会混在一起共同食用,这既是修行也是一种结缘。
产补辞苍别苍驳虫颈测别尘别颈测辞耻“锄耻辞测颈诲补颈产颈”。7测耻别24谤颈蝉丑补苍驳飞耻,锄丑辞苍驳箩耻驳补辞虫颈苍锄丑补辞办补颈诲辞苍驳蝉丑颈丑耻颈,辩耻别诲颈苍驳诲补苍驳谤颈虫颈补飞耻锄丑补辞办补颈诲别濒颈苍蝉丑颈驳耻诲辞苍驳诲补丑耻颈飞耻虫颈补辞。产耻驳耻辞,产别颈箩颈苍驳测颈锄补苍濒惫蝉丑颈蝉丑颈飞耻蝉耻辞锄丑耻谤别苍肠丑别苍测补苍驳濒惫蝉丑颈产颈补辞蝉丑颈:“迟颈测颈锄丑补辞办补颈濒颈苍蝉丑颈驳耻诲辞苍驳诲补丑耻颈蝉丑颈箩颈补苍蝉丑颈丑耻颈诲别蹿补诲颈苍驳锄丑颈辩耻补苍,诲辞苍驳蝉丑颈丑耻颈飞耻辩耻补苍迟辞苍驳驳耻辞箩耻别测颈诲别蹿补苍驳蝉丑颈辩耻别谤别苍辩颈飞耻虫颈补辞。”虫颈谤颈“濒颈补辞丑耻补苍驳诲颈”箩颈苍肠丑别苍驳“箩耻产补辞辫别苍”
这(窜丑别)样(驰补苍驳)优(驰辞耻)秀(齿颈耻)的(顿别)人(搁别苍)按(础苍)理(尝颈)来(尝补颈)说(厂丑耻辞),应(驰颈苍驳)该(骋补颈)自(窜颈)信(齿颈苍)张(窜丑补苍驳)扬(驰补苍驳)才(颁补颈)对(顿耻颈),可(碍别)这(窜丑别)位(奥别颈)连(尝颈补苍)打(顿补)针(窜丑别苍)都(顿耻)会(贬耻颈)掉(顿颈补辞)眼(驰补苍)泪(尝别颈)的(顿别)小(齿颈补辞)姑(骋耻)娘(狈颈补苍驳),又(驰辞耻)怎(窜别苍)么(惭别)会(贬耻颈)冒(惭补辞)这(窜丑别)么(惭别)大(顿补)的(顿别)风(贵别苍驳)险(齿颈补苍),只(窜丑颈)为(奥别颈)了(尝颈补辞)与(驰耻)偶(翱耻)像(齿颈补苍驳)有(驰辞耻)些(齿颈别)相(齿颈补苍驳)似(厂颈)呢(狈别)?
nongyeyinxingzhejianglishuilianduzhixinggongsiyewubujingli huyaya:tongguozheleizhongchangqidedaikuan,keyiyouxiaojianqingqiyezijinyali。muqianshifafangliao4000duowanyuan,tongshiwomenhuanzhudongjianfeirangli,balilvxiadiaoliao50gejidian,yiniankeyiweiqiyejieshengjin20wanyuandelixizhichu。worenwei,zhiyouyingshanghuanjing“zhuhaochao”,shanghaiguojijinrongzhongxinjianshecaineng“yinhaofeng”。shanghaishizi2018nianqi,meiniansuimonianchuduhuibushuchutaiyouhuayingshanghuanjingshishifangan,jinnianchu,yituichuyouhuayingshanghuanjing6.0banxingdongfangan。danwomenyekandao,yuniuyue、lundunxiangbi,shanghaiguojijinrongzhongxinjianshehuancunzaijiaodanengjicha,yixielingyudaerbuqiang、feierbuzhuang,zaigaishanshanghaiguojijinrongzhongxinyingshanghuanjingfangmian,muqianrengmianlinyixietongdian、nandianhedudian。biru,fazhifangmian,jinronglingyulifadeqianzhanxing、kexuexing、xietongxinghuanbugouqiang,jinrongfazhiyujinrongzhongxindeguojidiweirengbupipei。shuishoufangmian,jinrongqiyehegerenshuifurengranpiangao,shuishouzhengcehuanjingduihaiwaijinrongjigouhegaoduanrencaidecichangyinlihuanbuqiang。ESGlinianfangmian,womenrengranchuyutansuoheqibujieduan,shangweixingchengxitongdeESGfagui、zhidutixihetouzijizhi。
QB/T 5759-2022 番(Fan)茄(Qie)酱(Jiang)罐(Guan)头(Tou)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)含(Han)量(Liang)测(Ce)定(Ding) 高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)法(Fa)2023-10-20 08:36·xinpulai中(Zhong)华(Hua)人(Ren)民(Min)共(Gong)和(He)国(Guo)轻(Qing)工(Gong)行(Xing)业(Ye)标(Biao)准(Zhun)QB/T 5759-2022番(Fan)茄(Qie)酱(Jiang)罐(Guan)头(Tou)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)含(Han)量(Liang)测(Ce)定(Ding)高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)法(Fa)Determination of lycopene in canned tomato paste-HPLC method前(Qian)言(Yan)本(Ben)文(Wen)件(Jian)按(An)照(Zhao)GB/T1.1-2020《标(Biao)准(Zhun)化(Hua)工(Gong)作(Zuo)导(Dao)则(Ze)第(Di)1部(Bu)分(Fen): 标(Biao)准(Zhun)化(Hua)文(Wen)件(Jian)的(De)结(Jie)构(Gou)和(He)起(Qi)草(Cao)规(Gui)则(Ze)》的(De)规(Gui)定(Ding)起(Qi)草(Cao)。本(Ben)文(Wen)件(Jian)由(You)中(Zhong)国(Guo)轻(Qing)工(Gong)业(Ye)联(Lian)合(He)会(Hui)提(Ti)出(Chu)。本(Ben)文(Wen)件(Jian)由(You)全(Quan)国(Guo)食(Shi)品(Pin)工(Gong)业(Ye)标(Biao)准(Zhun)化(Hua)技(Ji)术(Shu)委(Wei)员(Yuan)会(Hui)罐(Guan)头(Tou)分(Fen)技(Ji)术(Shu)委(Wei)员(Yuan)会(Hui)(SACTC 64/SC2)归(Gui)口(Kou)。本(Ben)文(Wen)件(Jian)起(Qi)草(Cao)单(Dan)位(Wei):上(Shang)海(Hai)交(Jiao)通(Tong)大(Da)学(Xue)、中(Zhong)国(Guo)食(Shi)品(Pin)发(Fa)酵(Jiao)工(Gong)业(Ye)研(Yan)究(Jiu)院(Yuan)有(You)限(Xian)公(Gong)司(Si)、厦(Xia)门(Men)市(Shi)产(Chan)品(Pin)质(Zhi)量(Liang)监(Jian)督(Du)检(Jian)验(Yan)院(Yuan)、华(Hua)测(Ce)检(Jian)测(Ce)认(Ren)证(Zheng)集(Ji)团(Tuan)股(Gu)份(Fen)有(You)限(Xian)公(Gong)司(Si)、新(Xin)疆(Jiang)新(Xin)康(Kang)农(Nong)业(Ye)发(Fa)展(Zhan)有(You)限(Xian)公(Gong)司(Si)、中(Zhong)粮(Liang)新(Xin)疆(Jiang)屯(Tun)河(He)加(Jia)工(Gong)番(Fan)茄(Qie)工(Gong)程(Cheng)技(Ji)术(Shu)研(Yan)究(Jiu)中(Zhong)心(Xin)(有(You)限(Xian)公(Gong)司(Si))、新(Xin)疆(Jiang)冠(Guan)农(Nong)果(Guo)茸(Rong)股(Gu)份(Fen)有(You)限(Xian)公(Gong)司(Si)、山(Shan)西(Xi)仲(Zhong)测(Ce)计(Ji)量(Liang)研(Yan)究(Jiu)院(Yuan)有(You)限(Xian)公(Gong)司(Si)、漳(Zhang)州(Zhou)海(Hai)关(Guan)综(Zong)合(He)技(Ji)术(Shu)服(Fu)务(Wu)中(Zhong)心(Xin)、中(Zhong)国(Guo)罐(Guan)头(Tou)工(Gong)业(Ye)协(Xie)会(Hui)。本(Ben)文(Wen)件(Jian)主(Zhu)要(Yao)起(Qi)草(Cao)人(Ren):王(Wang)正(Zheng)武(Wu)、仇(Chou)凯(Kai)、方(Fang)荣(Rong)谦(Qian)、张(Zhang)维(Wei)、东(Dong)思(Si)源(Yuan)、柴(Chai)平(Ping)海(Hai)、高(Gao)静(Jing)、何(He)旻()霞(Xia)、谭(Tan)奇(Qi)敏(Min)、刘(Liu)中(Zhong)海(Hai)、吴(Wu)二(Er)豪(Hao)、王(Wang)登(Deng)飞(Fei)、晁(Zuo)曦(Zuo)、常(Chang)迪(Di)、童(Tong)跃(Yue)聪(Cong)、孙(Sun)字(Zi)、戚(Qi)晨(Chen)晨(Chen)、高(Gao)红(Hong)波(Bo)、崔(Cui)海(Hai)滨(Bin)、肖(Xiao)莉(Li)、杨(Yang)伟(Wei)敏(Min)、陈(Chen)泳(Yong)、陈(Chen)其(Qi)钢(Gang)、申(Shen)玥()琦(Zuo)、张(Zhang)国(Guo)玉(Yu)、周(Zhou)璇(Zuo)、张(Zhang)宇(Yu)聪(Cong)、赵(Zhao)娅(Zuo)鸿(Hong)。本(Ben)文(Wen)件(Jian)为(Wei)首(Shou)次(Ci)发(Fa)布(Bu)。1范(Fan)围(Wei)本(Ben)文(Wen)件(Jian)描(Miao)述(Shu)了(Liao)采(Cai)用(Yong)高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)法(Fa)测(Ce)定(Ding)番(Fan)茄(Qie)酱(Jiang)罐(Guan)头(Tou)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)含(Han)量(Liang)的(De)方(Fang)法(Fa)。本(Ben)文(Wen)件(Jian)适(Shi)用(Yong)于(Yu)采(Cai)用(Yong)高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)法(Fa)进(Jin)行(Xing)番(Fan)茄(Qie)酱(Jiang)罐(Guan)头(Tou)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)含(Han)量(Liang)的(De)测(Ce)定(Ding)。2规(Gui)范(Fan)性(Xing)引(Yin)用(Yong)文(Wen)件(Jian)下(Xia)列(Lie)文(Wen)件(Jian)中(Zhong)的(De)内(Nei)容(Rong)通(Tong)过(Guo)文(Wen)中(Zhong)的(De)规(Gui)范(Fan)性(Xing)引(Yin)用(Yong)而(Er)构(Gou)成(Cheng)本(Ben)文(Wen)件(Jian)必(Bi)不(Bu)可(Ke)少(Shao)的(De)条(Tiao)款(Kuan)。其(Qi)中(Zhong),注(Zhu)日(Ri)期(Qi)的(De)引(Yin)用(Yong)文(Wen)件(Jian),仅(Jin)该(Gai)日(Ri)期(Qi)对(Dui)应(Ying)的(De)版(Ban)本(Ben)适(Shi)用(Yong)于(Yu)本(Ben)文(Wen)件(Jian);不(Bu)注(Zhu)日(Ri)期(Qi)的(De)引(Yin)用(Yong)文(Wen)件(Jian),其(Qi)最(Zui)新(Xin)版(Ban)本(Ben)(包(Bao)括(Kuo)所(Suo)有(You)的(De)修(Xiu)改(Gai)单(Dan))适(Shi)用(Yong)于(Yu)本(Ben)文(Wen)件(Jian)。GB/T 6682分(Fen)析(Xi)实(Shi)验(Yan)室(Shi)用(Yong)水(Shui)规(Gui)格(Ge)和(He)试(Shi)验(Yan)方(Fang)法(Fa)。3术(Shu)语(Yu)和(He)定(Ding)义(Yi)本(Ben)文(Wen)件(Jian)没(Mei)有(You)需(Xu)要(Yao)界(Jie)定(Ding)的(De)术(Shu)语(Yu)和(He)定(Ding)义(Yi)。4原(Yuan)理(Li)试(Shi)样(Yang)在(Zai)碱(Jian)性(Xing)溶(Rong)液(Ye)中(Zhong)皂(Zao)化(Hua),经(Jing)盐(Yan)酸(Suan)溶(Rong)液(Ye)中(Zhong)和(He)至(Zhi)中(Zhong)性(Xing),用(Yong)有(You)机(Ji)溶(Rong)剂(Ji)萃(Zuo)取(Qu)试(Shi)样(Yang)中(Zhong)的(De)番(Fan)茄(Qie)红(Hong)素(Su),合(He)并(Bing)有(You)机(Ji)相(Xiang)并(Bing)进(Jin)行(Xing)浓(Nong)缩(Suo),经(Jing)C30色(Se)谱(Pu)柱(Zhu)分(Fen)离(Li),高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)紫(Zi)外(Wai)检(Jian)测(Ce)器(Qi)测(Ce)定(Ding),外(Wai)标(Biao)法(Fa)定(Ding)量(Liang)。5试(Shi)剂(Ji)除(Chu)另(Ling)有(You)说(Shuo)明(Ming)外(Wai),所(Suo)有(You)试(Shi)剂(Ji)均(Jun)为(Wei)分(Fen)析(Xi)纯(Chun),水(Shui)为(Wei)GBT 6682规(Gui)定(Ding)的(De)一(Yi)级(Ji)水(Shui)。5.1 甲(Jia)醇(Chun)(CH3OH):色(Se)谱(Pu)纯(Chun)。5.2 甲(Jia)基(Ji)叔(Shu)丁(Ding)基(Ji)醚(Mi)(C5H12O):色(Se)谱(Pu)纯(Chun)。5.3 二(Er)氯(Lv)甲(Jia)烷(Wan)(CH2Cl2)。5.4 浓(Nong)盐(Yan)酸(Suan)(HCl)。5.5 无(Wu)水(Shui)乙(Yi)醇(Chun)(C2H6O)。5.6 氯(Lv)化(Hua)钠(Na)(NaCl)。5.7 氢(Qing)氧(Yang)化(Hua)钠(Na)(NaOH)。5.8 2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)。5.9石(Shi)油(You)醚(Mi):沸(Fei)程(Cheng)60℃~90℃。5.10番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)品(Pin)(CAS 502-65-8):包(Bao)含(Han)13z-番(Fan)茄(Qie)红(Hong)素(Su)、9z-番(Fan)茄(Qie)红(Hong)素(Su)、全(Quan)反(Fan)式(Shi)-番(Fan)茄(Qie)红(Hong)素(Su)、5z-番(Fan)茄(Qie)红(Hong)素(Su)混(Hun)合(He)物(Wu),有(You)证(Zheng)标(Biao)准(Zhun)物(Wu)质(Zhi),纯(Chun)度(Du)大(Da)于(Yu)95%。5.11 氢(Qing)氧(Yang)化(Hua)钠(Na)溶(Rong)液(Ye)(0.5 mol/L):称(Cheng)取(Qu)20g (精(Jing)确(Que)至(Zhi)0.01g)氢(Qing)氧(Yang)化(Hua)钠(Na)(5.7)于(Yu)500 mL烧(Shao)杯(Bei)中(Zhong),加(Jia)适(Shi)量(Liang)水(Shui)溶(Rong)解(Jie),待(Dai)溶(Rong)液(Ye)恢(Hui)复(Fu)至(Zhi)室(Shi)温(Wen)后(Hou),转(Zhuan)移(Yi)至(Zhi)1 000 mL容(Rong)量(Liang)瓶(Ping)中(Zhong)定(Ding)容(Rong)至(Zhi)刻(Ke)度(Du)。5.12盐(Yan)酸(Suan)溶(Rong)液(Ye)(0.6 mo/L):准(Zhun)确(Que)量(Liang)取(Qu)25.0mL浓(Nong)盐(Yan)酸(Suan)(5.4)于(Yu)500 mL容(Rong)量(Liang)瓶(Ping)中(Zhong),用(Yong)水(Shui)稀(Xi)释(Shi)并(Bing)定(Ding)容(Rong)至(Zhi)刻(Ke)度(Du)。5.13 2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)-二(Er)氯(Lv)甲(Jia)烷(Wan)溶(Rong)液(Ye)(5g/L):准(Zhun)确(Que)称(Cheng)取(Qu)0.5g(精(Jing)确(Que)到(Dao)0.01 g)2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)(5.8)于(Yu)烧(Shao)瓶(Ping)中(Zhong)加(Jia)适(Shi)量(Liang)二(Er)氯(Lv)甲(Jia)烷(Wan)(5.3)溶(Rong)解(Jie),转(Zhuan)移(Yi)至(Zhi)100 mL棕(Zong)色(Se)容(Rong)量(Liang)瓶(Ping)中(Zhong),用(Yong)二(Er)氯(Lv)甲(Jia)烷(Wan)(5.3)定(Ding)容(Rong)至(Zhi)刻(Ke)度(Du)。5.14番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)储(Chu)备(Bei)溶(Rong)液(Ye)(100 mg/L):分(Fen)别(Bie)准(Zhun)确(Que)称(Cheng)取(Qu)5 mg(精(Jing)确(Que)至(Zhi)0.1 mg)番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)晶(Jing)(5.10)和(He)10mg2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)(5.8)(精(Jing)确(Que)至(Zhi)0.1 mg)于(Yu)烧(Shao)杯(Bei)中(Zhong),用(Yong)少(Shao)量(Liang)二(Er)氯(Lv)甲(Jia)烷(Wan)(5.3)溶(Rong)解(Jie),完(Wan)全(Quan)转(Zhuan)移(Yi)至(Zhi)50 mL棕(Zong)色(Se)容(Rong)量(Liang)瓶(Ping)中(Zhong)并(Bing)定(Ding)容(Rong)刻(Ke)度(Du),-20℃避(Bi)光(Guang)保(Bao)存(Cun),24 h内(Nei)可(Ke)使(Shi)用(Yong)。5.15番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)溶(Rong)液(Ye): 量(Liang)取(Qu)1.0 mL (VA)番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)储(Chu)备(Bei)溶(Rong)液(Ye)(5.14),置(Zhi)于(Yu)50mL (VB)棕(Zong)色(Se)容(Rong)量(Liang)瓶(Ping)中(Zhong),加(Jia)入(Ru)5.0 mL无(Wu)水(Shui)乙(Yi)醇(Chun)(5.5)和(He)5.0ml 2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)-二(Er)氯(Lv)甲(Jia)烷(Wan)溶(Rong)液(Ye)(5.13),用(Yong)石(Shi)油(You)醚(Mi)(5.9)定(Ding)容(Rong),混(Hun)匀(Yun)后(Hou)将(Jiang)溶(Rong)液(Ye)置(Zhi)于(Yu)1 cm比(Bi)色(Se)皿(Min)中(Zhong),以(Yi)石(Shi)油(You)醚(Mi)(5.9)作(Zuo)空(Kong)白(Bai)对(Dui)照(Zhao),用(Yong)紫(Zi)外(Wai)-可(Ke)见(Jian)光(Guang)分(Fen)光(Guang)光(Guang)度(Du)计(Ji)在(Zai)472 nm处(Chu)测(Ce)定(Ding)吸(Xi)光(Guang)度(Du)。吸(Xi)光(Guang)度(Du)应(Ying)控(Kong)制(Zhi)在(Zai)0.3~0.7,否(Fou)则(Ze)应(Ying)调(Diao)整(Zheng)溶(Rong)液(Ye)浓(Nong)度(Du),再(Zai)重(Zhong)新(Xin)测(Ce)定(Ding)吸(Xi)光(Guang)度(Du)。番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)溶(Rong)液(Ye)质(Zhi)量(Liang)浓(Nong)度(Du)ρst,按(An)公(Gong)式(Shi)(1)计(Ji)算(Suan):式(Shi)中(Zhong):ρst——番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)溶(Rong)液(Ye)质(Zhi)量(Liang)浓(Nong)度(Du),单(Dan)位(Wei)为(Wei)毫(Hao)克(Ke)每(Mei)升(Sheng)(mg/L);Amax——番(Fan)茄(Qie)红(Hong)素(Su)溶(Rong)液(Ye)在(Zai)472 nm附(Fu)近(Jin)的(De)最(Zui)大(Da)吸(Xi)收(Shou)波(Bo)长(Chang)处(Chu)测(Ce)得(De)的(De)吸(Xi)光(Guang)度(Du)值(Zhi);D——稀(Xi)释(Shi)因(Yin)子(Zi)(VE/VA);10 000——浓(Nong)度(Du)换(Huan)算(Suan)因(Yin)子(Zi);3 450——番(Fan)茄(Qie)红(Hong)素(Su)的(De)吸(Xi)光(Guang)系(Xi)数(Shu)。5.16番(Fan)茄(Qie)红(Hong)素(Su)系(Xi)列(Lie)标(Biao)准(Zhun)工(Gong)作(Zuo)液(Ye):分(Fen)别(Bie)准(Zhun)确(Que)量(Liang)取(Qu)0mL、0.05 mL、0.1 mL、0.2 mL、0.4 mL、0.8 mL、1.0 mL番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)准(Zhun)储(Chu)备(Bei)溶(Rong)液(Ye)(5.14),用(Yong)二(Er)氯(Lv)甲(Jia)烷(Wan)(5.3)定(Ding)容(Rong)至(Zhi)10 mL,得(De)到(Dao)质(Zhi)量(Liang)浓(Nong)度(Du)为(Wei)0μg/mL、0.5μg/mL、1.0μg/mL、2.0μg/mL、4.0μg/mlL、8.0μgmL、10.0μg/mL的(De)番(Fan)茄(Qie)红(Hong)素(Su)系(Xi)列(Lie)标(Biao)准(Zhun)工(Gong)作(Zuo)溶(Rong)液(Ye),现(Xian)配(Pei)现(Xian)用(Yong)。番(Fan)茄(Qie)红(Hong)素(Su)系(Xi)列(Lie)标(Biao)准(Zhun)工(Gong)作(Zuo)液(Ye)浓(Nong)度(Du)按(An)照(Zhao)标(Biao)定(Ding)的(De)实(Shi)际(Ji)浓(Nong)度(Du)计(Ji)算(Suan),标(Biao)准(Zhun)曲(Qu)线(Xian)浓(Nong)度(Du)范(Fan)围(Wei)可(Ke)根(Gen)据(Ju)标(Biao)定(Ding)浓(Nong)度(Du)适(Shi)当(Dang)调(Diao)整(Zheng)。6仪(Yi)器(Qi)和(He)材(Cai)料(Liao)6.1高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)仪(Yi): 配(Pei)备(Bei)紫(Zi)外(Wai)检(Jian)测(Ce)器(Qi)(UV)。6.2电(Dian)子(Zi)天(Tian)平(Ping): 感(Gan)量(Liang)0.1 mg和(He)0.01 g。6.3磁(Ci)力(Li)搅(Jiao)拌(Ban)器(Qi)。6.4旋(Xuan)转(Zhuan)蒸(Zheng)发(Fa)仪(Yi)。6.5涡(Wo)旋(Xuan)混(Hun)合(He)器(Qi)。6.6 振(Zhen)荡(Dang)器(Qi)。6.7 pH计(Ji)。6.8高(Gao)速(Su)离(Li)心(Xin)机(Ji)。6.9有(You)机(Ji)微(Wei)孔(Kong)滤(Lv)膜(Mo):孔(Kong)径(Jing)0.45μm。7分(Fen)析(Xi)步(Bu)骤(Zhou)7.1 样(Yang)品(Pin)前(Qian)处(Chu)理(Li)称(Cheng)取(Qu)0.5g样(Yang)品(Pin)(精(Jing)确(Que)至(Zhi)0.01g)于(Yu)50mL离(Li)心(Xin)管(Guan)中(Zhong),加(Jia)入(Ru)5 mL氢(Qing)氧(Yang)化(Hua)钠(Na)溶(Rong)液(Ye)(5.11)溶(Rong)解(Jie)混(Hun)匀(Yun)(涡(Wo)旋(Xuan)混(Hun)合(He)器(Qi)),25℃室(Shi)温(Wen)皂(Zao)化(Hua)30 min,分(Fen)别(Bie)加(Jia)入(Ru)15mL水(Shui)和(He)2g氯(Lv)化(Hua)钠(Na)(5.6),用(Yong)振(Zhen)荡(Dang)器(Qi)充(Chong)分(Fen)振(Zhen)荡(Dang)使(Shi)溶(Rong)液(Ye)分(Fen)散(San)均(Jun)匀(Yun),用(Yong)盐(Yan)酸(Suan)溶(Rong)液(Ye)(5.12)中(Zhong)和(He)至(Zhi)pH=7.0(pH=7±0.1,20℃,pH计(Ji)),加(Jia)入(Ru)20 mL2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)-二(Er)氯(Lv)甲(Jia)烷(Wan)溶(Rong)液(Ye)(5.13),用(Yong)振(Zhen)荡(Dang)器(Qi)振(Zhen)荡(Dang)10 min,置(Zhi)于(Yu)4 000 r/min高(Gao)速(Su)离(Li)心(Xin)机(Ji)离(Li)心(Xin)3 min,用(Yong)玻(Bo)璃(Li)吸(Xi)管(Guan)将(Jiang)下(Xia)层(Ceng)有(You)机(Ji)溶(Rong)液(Ye)全(Quan)部(Bu)转(Zhuan)移(Yi)至(Zhi)250 mL圆(Yuan)底(Di)烧(Shao)瓶(Ping)中(Zhong),在(Zai)上(Shang)层(Ceng)水(Shui)相(Xiang)和(He)残(Can)渣(Zha)中(Zhong)加(Jia)入(Ru)20 mL 2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)-二(Er)氯(Lv)甲(Jia)烷(Wan)溶(Rong)液(Ye)(5.13),重(Zhong)复(Fu)以(Yi)止(Zhi)操(Cao)作(Zuo)3次(Ci),直(Zhi)至(Zhi)水(Shui)相(Xiang)和(He)残(Can)渣(Zha)无(Wu)色(Se),合(He)并(Bing)有(You)机(Ji)相(Xiang)。将(Jiang)圆(Yuan)底(Di)烧(Shao)瓶(Ping)中(Zhong)的(De)有(You)机(Ji)溶(Rong)液(Ye)在(Zai)40℃下(Xia)用(Yong)旋(Xuan)转(Zhuan)蒸(Zheng)发(Fa)仪(Yi)浓(Nong)缩(Suo)旋(Xuan)蒸(Zheng)至(Zhi)约(Yue)2 mL,将(Jiang)溶(Rong)液(Ye)转(Zhuan)移(Yi)至(Zhi)10 mL的(De)棕(Zong)色(Se)容(Rong)量(Liang)瓶(Ping)中(Zhong),用(Yong)2,6-二(Er)叔(Shu)丁(Ding)基(Ji)对(Dui)甲(Jia)酚(Fen)(BHT)仁(Ren)氯(Lv)甲(Jia)烷(Wan)溶(Rong)液(Ye)(5.13)润(Run)洗(Xi)圆(Yuan)底(Di)烧(Shao)瓶(Ping)至(Zhi)少(Shao)3次(Ci),合(He)并(Bing)润(Run)洗(Xi)液(Ye)并(Bing)定(Ding)容(Rong),滤(Lv)液(Ye)经(Jing)0.45μm有(You)机(Ji)微(Wei)孔(Kong)滤(Lv)膜(Mo)过(Guo)滤(Lv),用(Yong)于(Yu)液(Ye)相(Xiang)色(Se)谱(Pu)检(Jian)测(Ce)。7.2 仪(Yi)器(Qi)参(Can)考(Kao)条(Tiao)件(Jian)7.2.1 色(Se)谱(Pu)柱(Zhu):C30色(Se)谱(Pu)柱(Zhu)(4.6 mm×250 mm,5μm)或(Huo)性(Xing)能(Neng)相(Xiang)当(Dang)者(Zhe)。7.2.2流(Liu)动(Dong)相(Xiang):A为(Wei)甲(Jia)醇(Chun),B为(Wei)甲(Jia)基(Ji)叔(Shu)丁(Ding)基(Ji)醚(Mi):三(San)乙(Yi)胺(An)-100:0.05(体(Ti)积(Ji)比(Bi))7.2.3 流(Liu)速(Su):1.0 mL/min.7.2.4 检(Jian)测(Ce)波(Bo)长(Chang):472 nm。7.2.5 柱(Zhu)温(Wen):30℃。7.2.6 进(Jin)样(Yang)量(Liang):10μL。7.2.7 梯(Ti)度(Du)洗(Xi)脱(Tuo)条(Tiao)件(Jian),见(Jian)表(Biao)1。表(Biao)1 梯(Ti)度(Du)洗(Xi)脱(Tuo)程(Cheng)序(Xu)表(Biao)时(Shi)间(Jian)/minA/(%)B/(%)080201565352045552435653032683720803910904210904380204880207.3 定(Ding)性(Xing)分(Fen)析(Xi)分(Fen)别(Bie)将(Jiang)番(Fan)茄(Qie)红(Hong)素(Su)系(Xi)列(Lie)标(Biao)准(Zhun)工(Gong)作(Zuo)液(Ye)(5.16)和(He)经(Jing)7.1制(Zhi)备(Bei)的(De)试(Shi)样(Yang)溶(Rong)液(Ye)进(Jin)样(Yang)测(Ce)定(Ding),以(Yi)番(Fan)茄(Qie)红(Hong)素(Su)的(De)保(Bao)留(Liu)时(Shi)间(Jian)定(Ding)性(Xing),定(Ding)性(Xing)色(Se)谱(Pu)图(Tu)见(Jian)附(Fu)录(Lu)A。7.4 外(Wai)标(Biao)法(Fa)定(Ding)量(Liang)将(Jiang)7.1制(Zhi)备(Bei)的(De)试(Shi)样(Yang)注(Zhu)入(Ru)高(Gao)效(Xiao)液(Ye)相(Xiang)色(Se)谱(Pu)仪(Yi),测(Ce)定(Ding)试(Shi)样(Yang)中(Zhong)各(Ge)组(Zu)分(Fen)谱(Pu)峰(Feng)面(Mian)积(Ji),以(Yi)番(Fan)茄(Qie)红(Hong)素(Su)标(Biao)定(Ding)后(Hou)的(De)系(Xi)列(Lie)标(Biao)准(Zhun)工(Gong)作(Zuo)液(Ye)浓(Nong)度(Du)为(Wei)横(Heng)坐(Zuo)标(Biao),以(Yi)番(Fan)茄(Qie)红(Hong)素(Su)各(Ge)异(Yi)构(Gou)体(Ti)的(De)峰(Feng)面(Mian)积(Ji)和(He)为(Wei)纵(Zong)坐(Zuo)标(Biao),绘(Hui)制(Zhi)标(Biao)准(Zhun)曲(Qu)线(Xian),由(You)标(Biao)准(Zhun)工(Gong)作(Zuo)曲(Qu)线(Xian)计(Ji)算(Suan)试(Shi)样(Yang)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)的(De)含(Han)量(Liang)。7.5 结(Jie)果(Guo)计(Ji)算(Suan)将(Jiang)样(Yang)品(Pin)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)的(De)含(Han)量(Liang)按(An)公(Gong)式(Shi)(2)计(Ji)算(Suan):式(Shi)中(Zhong):X——桃(Tao)合(He)一(Yi)样(Yang)品(Pin)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)的(De)含(Han)量(Liang),单(Dan)位(Wei)为(Wei)毫(Hao)克(Ke)每(Mei)百(Bai)克(Ke)(mg/100g);ρ——待(Dai)测(Ce)溶(Rong)液(Ye)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)总(Zong)质(Zhi)量(Liang)浓(Nong)度(Du),单(Dan)位(Wei)为(Wei)微(Wei)克(Ke)每(Mei)毫(Hao)升(Sheng)(μg/mL);V——溶(Rong)液(Ye)的(De)定(Ding)容(Rong)体(Ti)积(Ji),单(Dan)位(Wei)为(Wei)毫(Hao)升(Sheng)(mL);100——换(Huan)算(Suan)因(Yin)子(Zi);m——样(Yang)品(Pin)质(Zhi)量(Liang),单(Dan)位(Wei)为(Wei)克(Ke)(g);1000——换(Huan)算(Suan)因(Yin)子(Zi)。测(Ce)定(Ding)结(Jie)果(Guo)取(Qu)两(Liang)次(Ci)测(Ce)定(Ding)的(De)算(Suan)术(Shu)平(Ping)均(Jun)值(Zhi),计(Ji)算(Suan)结(Jie)果(Guo)保(Bao)留(Liu)至(Zhi)小(Xiao)数(Shu)点(Dian)后(Hou)2位(Wei)。7.6精(Jing)密(Mi)度(Du)在(Zai)重(Zhong)复(Fu)性(Xing)条(Tiao)件(Jian)下(Xia)获(Huo)得(De)的(De)两(Liang)次(Ci)独(Du)立(Li)测(Ce)定(Ding)结(Jie)果(Guo)的(De)绝(Jue)对(Dui)差(Cha)值(Zhi)不(Bu)应(Ying)超(Chao)过(Guo)其(Qi)算(Suan)术(Shu)平(Ping)均(Jun)值(Zhi)的(De)10%。7.7检(Jian)出(Chu)限(Xian)和(He)定(Ding)量(Liang)限(Xian)本(Ben)文(Wen)件(Jian)中(Zhong)番(Fan)茄(Qie)红(Hong)素(Su)的(De)检(Jian)出(Chu)限(Xian)为(Wei)0.30 mg/100g,定(Ding)量(Liang)限(Xian)为(Wei)1.00 mg/100g。转(Zhuan)载(Zai)自(Zi):http://www.xpl-hplc.com/enstyle/articleinfo_11793215.html
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当然了王朔也曾经说过马未都说的很多话实际上是有一定局限性的这样的局限性也会导致认不清一些现实就比如现在裁员都是要依法依规进行的而且有明确的规定如果意识不到这一点的话就容易把事情闹大单方面的裁员实际上是没有考虑清楚要承担的法律后果不过具体是怎么回事还是应该法律程序走完再说不排除有其他可能因为现在仅仅是一面之词我们还是应该理性客观不站队学会独立思考