最新免费电影冲热门电影在线观看冲巨人影院含有“巨熟乳波霸中文字幕”等内容的视频在线观看
她的感情态度和生活方式，与其说是叛逆，不如说是对自由的追求。或许，这正是她在缺乏家庭温暖的环境中，为自己寻找的一种情感补偿方式。
2024年12月21日，对于那些频繁做这些动作的人，不妨从心理学的角度出发，审视自己的内心世界，寻求更健康的心理调节方法。
最新免费电影冲热门电影在线观看冲巨人影院含有“巨熟乳波霸中文字幕”等内容的视频在线观看
这些年她也在反思过去的那些经历让她学会了怎么把自己的尊严一片片捡回来重新拼好
懵懵懂懂的少女心思在李娟笔下显现，现实中李娟和麦西拉没有在一起，甚至可以说几乎还没有开始，就匆匆地结束了。茭白。茭白是夏季的应季蔬菜，它含有大量的水分和纤维，非常适合在炎热的天气中食用。茭白性凉，可以帮助清热解毒，对于夏天常见的疾病如热伤风和喉咙痛有一定的缓解作用。在烹饪时，可以将茭白做成汤或者凉拌，这样既能保持其营养价值，也能增加食欲。
虫颈补迟颈补苍诲补辞濒颈补辞，罢虫耻诲别蝉丑耻蹿耻产补颈诲补辩颈别箩颈苍驳箩颈蝉丑辞耻诲补辞驳耻补苍驳诲补苍惫蝉丑别苍驳诲别虫颈补颈，虫颈补苍驳虫颈苍诲补箩颈补锄补颈测补苍测补苍虫颈补谤颈办别测颈产耻尘补颈辫颈补辞濒颈补苍驳诲别辩耻苍锄颈，诲补苍蝉丑颈测颈诲颈苍驳产耻苍别苍驳尘别颈测辞耻罢虫耻。诲耻颈测耻40蝉耻颈诲别苍惫虫颈苍驳濒补颈蝉丑耻辞，虫颈补箩颈诲别肠丑耻补苍诲补产耻箩颈苍测补辞蝉丑耻蝉丑颈迟辞耻辩颈，驳别苍驳测补辞锄丑补苍虫颈补苍肠丑耻肠丑别苍驳蝉丑耻苍惫虫颈苍驳诲别测辞耻测补测耻锄耻辞濒颈。罢虫耻锄耻辞飞别颈虫颈补箩颈诲别产颈产别颈诲补苍辫颈苍，谤耻丑别肠丑耻补苍诲补肠补颈苍别苍驳箩颈蝉丑颈蝉丑补苍驳测辞耻诲别迟颈苍别？别谤虫颈苍苍别苍驳测耻补苍肠丑别蝉丑颈诲补颈，锄补颈谤耻补苍箩颈补苍办辞苍驳锄丑颈虫颈补，诲耻辞驳别诲颈补苍箩颈箩颈补苍驳诲辞苍驳濒颈肠丑耻补苍蝉丑耻诲补辞锄丑颈诲颈苍驳肠丑别濒耻苍，蝉丑颈虫颈补苍蝉颈濒耻苍蹿别苍产耻蝉丑颈辩耻诲辞苍驳，锄丑别测补苍驳箩颈耻谤补辞驳耻辞濒颈补辞蝉颈濒耻苍辩耻诲辞苍驳虫颈迟辞苍驳丑别肠丑补蝉耻箩颈驳辞耻诲别箩颈蝉丑耻尘别苍箩颈补苍。
需(齿耻)要(驰补辞)指(窜丑颈)出(颁丑耻)的(顿别)是(厂丑颈)，新(齿颈苍)华(贬耻补)叁(厂补苍)的(顿别)资(窜颈)产(颁丑补苍)负(贵耻)债(窜丑补颈)率(尝惫)达(顿补)到(顿补辞)七(蚕颈)成(颁丑别苍驳)，截(闯颈别)至(窜丑颈)2022年(狈颈补苍)末(惭辞)，新(齿颈苍)华(贬耻补)叁(厂补苍)的(顿别)总(窜辞苍驳)资(窜颈)产(颁丑补苍)达(顿补)330.44亿(驰颈)元(驰耻补苍)，负(贵耻)债(窜丑补颈)合(贬别)计(闯颈)232.88亿(驰颈)元(驰耻补苍)。
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repeat extensin proteins regulate plant salt tolerance in Arabidopsis”yiwenzhong，zuozheweiliaoyanjiuLRXdanbaizaishengchangdiaokonghenaiyanxingzhongdezuoyongjizhi，shiyong35S::LRX3-YFP-HA、35S::LRX4-YFP-HAhe35S::LRX5-YFP-HAzhuanjiyinzhiwujinxingliaomianyichendian-zhipu（IP-MS）fenxi，yiquedingLRXsdeqianzaixianghuzuoyongdanbai。tongshiyibiaoda35S::GFPdezhuanjiyinzhizhuzuoweiduizhao。jieguofaxian，zaiLRXzhuanjiyinzhiwudeIP-MSyangbenzhongjiandingchuliao4gexitongfayuxiangguandeRALFduozuo：RALF22、RALF23、RALF24heRALF31，danzaiduizhaoyangbenzhongmeiyoufaxian（tu1）。houmiandeshiyanwoxiangdabufenxiaohuobanduyijingzhidaoshishimeliao。meicuo！jiushiliyongdianduidianyanzhengdanbaihuzuodefangfaduiIP-MSjiandingdedaodeLRXdehuzuodanbaijinxingyanzheng，zhegetaoluxiangxindajiayijingshuxidebunengzaishuxiliao，suoyibuqingchudexiaohuobanyidingyaojixialaio！tu1 zaiIP-MSjiancezhongjiandingchudeLRXheRALFdanbaideduozuo(Zhao et al., 2018)。cong35S::LRX3-YFP-HA、35S::LRX4-YFP-HAhe35S::LRX5-YFP-HAzhuanjiyinzhizhuzhongtiqudanbai。yiguobiaodakongzaiGFPdezhuanjiyinzhizhuweiduizhao。fenlidedanbaiyukangGFPkangtifuyuguoye。yongzhipufaduimianyichendianyangpinjinxingfenxi。meigedanbaizhisuojiandingdeduozuodeshuliangbeixianshichulai，pozhehao“-”biaoshizuomeiyoubeishibie。xiaoyuanzuozuo：ruguowomenxiangyaoyanjiuyigeweizhidanbaidehuzuodanbaishishime，chuliaoxiangdaojiaomushuangzashaikudeshiyanzhiwai，IP-MSyeshiwomenyinggaixiangdaodefangfa。IP-MSbujinnengdedaoyujiaomushuangzashaikuleisidejieguo，bingqiehuanjuyouyixiayoudian：1、IP-MSshaixuandeshitianranzhuangtaixiadedanbai（meiyoupohuaidanbaidejiegou）huzuo，zaiyidingchengdushanggengnengfanyingshengwutineidezhenshiqingkuang；2、buyongxiangjiaomushuangzashaikunayang，danxinyouerdanbaihuicunzaizijihuozuoyong。yinci，yihouzaiyanjiuyigeweizhidanbaidehuzuodanbaishikeyishouxiankaolvIP-MSo！zhipudingliangfenxichuliaobiaoxingbiaodahedanbaizhitexingdechubujiandingwai，danbaizhizuxuefenxizhongdeyigeguanjiancanshushiduiganxingqudedanbaizhijinxingdingliangdenengli。dayueershinianqian，danbaizhizuxuezaihendachengdushanghuanshiyimendingxingxueke。dianxingdedanbaizhizuxueshiyanjieguoshizaiyigegeidingdezuzhihuodanbaizhifuhewuzhongjiandingchudanbaizhideliebiao，ermeiyourenheguanyufengdu、fenbuhuohuaxuejiliangxuedejinyibuxinxi。xiangbizhixia，nageshihoudingliangcelueyibeiguangfanyingyongyuweizhenliehuodingliangPCRdejiyinbiaodafenxi，tebieshizaizhiwuzhong，daguimojiyinzuxuedecedingweizhiwufayuheshenglidexuduofangmiantigongliaoxindejianjie。raner，meidefanyinghexinhaotongluzuizhongqujueyudanbaizhidehuoxing。danbaizhideshuliangshoudanbaizhihechenghejiangjiedediaokong，kenengyuzhuanludiaokongmeiyoutaidadeguanxi(Piques et al., 2022)。ciwai，fanyihouxiushi、yigoutihejianjiebianyitibunengjintongguofenxizhuanlubendefengdulaihuoqu。yinci，weiliaoduiyixieshengwuxuexianxiangjinxingjieshi，danbaidingliangfenxishibiyaode。zhipudechuxianweidanbaizhizufuzaxingfenxitigongliaojishukuangjia。dingliangdanbaizhizuxuekeyifenweixiangduidinglianghejueduidingliang。xiangduidingliangzhizaiyanjiubutongtiaojianxiadanbaizhizubiaodadechayi，erjueduidingliangshizhihuodedanbaizhideteyixingbiaodashuiping。jueduidingliangxuyaoshiyongbiaozhunhuadecankaoyangpin。xiaoyuanzaixiabiaozhongliechuliaoyixiechangjiandezhipudingliangfangfa，dajiayouxingqukeyishenruliaojie，zhelibuzuojutijieshaoo！biao1 danbaizhidingliangzuxuebutonggongzuoliuchengdejishucanshugaishuzhu：1zhiyoutongguoyujiabiaojideyizhibiaozhunpinjinxingxiangduibijiao，cainengshixianjueduidingliang。2jueduidingliangzhinengtongguojingyantezhengheshiyongyangpinzhongdanbaizhidefenzilianghezongdanbaizhiliangdefanjisuanlaijinxing。MS2：zhipufaduizuoliziposuihouchanshengdesuoyoulizijinxingjiance。pianduanlizisaomiaoyechengweiMS2saomiaohuoMS/MSsaomiao。zhipufaquedingdanbaifanyihouxiushifanyihouxiushi（PTMs）shitongguodanbaishuijieliejiehuojiangxiushijituangongjiajiadaoyigehuoduogeanjisuanshangergaibiandanbaizhixingzhidejiagongguocheng。jinjingenjufengdudebianhualaimiaoshudanbaizhidegongnengduiliaojiedanbaizhizushifeichangyouxiande，yinweixuduodanbaizhidehuoxingshiyouPTMsdiaojiede，erPTMskenengbunengzhunquedifanyingdanbaizhifengdudebianhua。zhejiushiweishimexuduoyongzaidanbaizhizufanweineibiaozhenghedingliangdanbaizhiheduozuodezhipujishuyijingbeiyingyongyubiaozhengfanyihouxiushidedanbaizhideyuanyin。PTMsyouduozhongleixing，baokuolinsuanhua、fansuhua、yizuohua、tangjihuadeng。zhipufabeirenweishidanbaizhixiushifenxideyigeguanjianjishu，yinweitakeyitigongyouguandanbaizhixiushidetongyongxinxierwuxuxianzhidaoxiushiweidiandeweizhi。zishangerxia，zizhongershanghezixiaershangdefangfashijiyuMSdePTMfenxidesanzhongzhuyaocelue。zhenduizhipudanbaidingliangyuzhipuquedingfanyidanbaifanyihouxiushi，xiaoyuanxianyongyigelizigeidajiajinxingjiandanshuoming，houqizaiduizhebufenneirongjinxingxiangxijiangjie，yinweigaibufenzhankailaijiangyeshiyigedagongchenga！lizilailiao：moshizhiwuninanjiebujingaibianliaowomenduizhiwushengwuxuederenshi，huanyingxiangliaoshengmingkexuedexuduoqitalingyu。laizininanjiedeyanjiujieguoyeweizuowudezhongyaonongyixingzhuangyanjiutigongliaosilu。ninanjiedejiyinzuzai20nianqianbeicexu，zinayihou，renmenzaijiyinzuhebiaoguanjiyinzushuipingshangfenxiliaoshubaizhongziranbianyi。xiangbizhixia，ninanjiedanbaizhizuzuoweidaduoshushengmingguochengdezhuyaozhixingzhe，qitezhengyuanmeiyounamequanmian。weiliaojiejuezheyichaju，zai“Mass-spectrometry-based draft of the Arabidopsis proteome”yiwenzhong，zuozheshiyongliaozuixianjindezhipuheRNAcexu（RNA-seq）fenxi，huodeliaowomensuozhidediyigeninanjiezonghedanbaizhizu、lindanbaizhizuhezhuanluzutupu。zhegefengfudefenziziyuankeyiyonglaitansuodangedanbaizhidegongnenghuokuayuejigezuxueshuipingdezhenggetujing。tu2 zuzhituheduozushujuji(Mergner et al., 2020)。a. fenxidezuzhiyangbenshiyitu，genjuxingtaixuefenzuzhuose（suoxiedingyizaibzhong）：hua（qianhuise）；zhongzi（shenzongse）；huafen（huangse）；jing（shenlvse）；ye（qianlvse）；gen（shenhuise）；shuiguo（qianzongse）；yushangzuzhi（hongse）；xibaopeiyang（lanse）。b. suoyouzuzhidedanbaizhi、P-weidianhezhuanlushuipingdeshibieshu（n=1geceliangzhi）。xuxianbiaoshizaisuoyouzuzhizhongjiancedaodehexindanbai、P-weidianhuozhuanlubendeshuliang。zuzhizengqiangdedanbaizhihuozhuanlubenyongjiaoshendeyansebiaoji。juyougaokexinduanjisuandingweideP-weidian（Ileiweidian；dayu0.75dedingweigailv）yongfensebiaoshi；mohudeweidiandingweiyongzisebiaoshi。zaiyigezuzhizhongteyixingjiancedaodeP-weidiandeshuliangyongyuanquanbiaoshi。c. yuAraport11xiangbi，zhuanluzu、danbaizhizuhelinsuanhuadanbaizhizushujujizhongjiandingchudejiyinweidianzongshuhezhongdiebufen（zuo），yijijiandingchudeP-weidianzongshuheIleiweidiandebili（you）。tongguozhegelizikeyikandao，zhipubujinkeyidedaodanbaishuipingdedingliangshuju，huankeyijiandinglinsuanhuaweidian，dedaolinsuanhuadanbaizhizushujuji。biqiRNA-seq，zhipushibushiyaolihaideduo，chuliaojiandinglinsuanhua，qitadedanbaifanyihouxiushiyekeyitongguozhipujinxingjianding，yaoxiangliaojiegengduo，qingguanzhuboxiaoyuanhouqidewenzhang！zhipuyudaixiedaixiezuxueshiyigexiangduijiaoxindeyanjiulingyu，raner，zicong1998nianOliverhetadehezuozheyinrudaixiezuzhegeshuyuhou，youguanzheyizhutidechubanwuchengzhishujizengchang。yudongwuxiangbi，zhiwuhanyoufeichangfengfudedaixiewu；zhiwuzhongdaixiewudezongshugujizai20wanhuogengduo(K. Oksman-Caldentey and K. Saito, 2005)。zhengyinruci，zhiwudaixiezuxuezaixuduolingyudedaoliaoguangfandeyingyong，ruyongyufenleixuehuoshengwuhuaxue，mudejiyinhuoshengtaixingzhiwenfenxi，tubiantihezhuanjiyinzhiwuyuqiyeshengxingdebijiao，waibucijiduidaixiechanwupudeyingxiang，zhiwuyucaoshidongwudexianghuzuoyong、fayuguocheng、yaocaizhiliangkongzhijiyaoyongzhiwuhuoxingceding(Wolfender et al., 2013)deng。youyuzhiwudaixiezuxuedeyingyongshifeichangduoyanghuade，yinci，yongyufenxizhiwudaixiezuxuedejishuyeshiduoyanghuade。lianyongfangfa，ruzhipuyuqixiangsepuhuoyexiangsepu（GC-MSheLC-MS）、zhijiezhushezhipu（DIMS）、hecigongzhen（NMR）、maoxiguandianyongzhipu（CE-MS）deng。xiamianboxiaoyuanyaogeidajiajieshaodeshiyizhongbijiaoxindegongzuoliucheng，gailiuchengyou2019nianfabiaozaiThe Plant Journalshangdeyipiantiwei“Comprehensive mass spectrometry-guided phenotyping of plant specialized metabolites reveals metabolic diversity in the cosmopolitan plant family Rhamnaceae”dewenzhangzhongtichu，wenzhangjieshaoliaogailiuchengshiyizhongkekuozhandebanzidonghuafangfa，tongguozhenghejizhongjisuanzhipu/zhipushujufenxifangfalaijiangzhiwudaixiechanwudeduoyangxinghefenbujinxingshuzihuazhanshi。tu3 liyongchuanlianzhipu（MS/MS）duizhiwuzhuanyongdaixiewujinxingbiaoxingfenxideshujufenxigongzuoliucheng(Kang et al., 2019)。liyongchuanlianzhipujinxingguangpuxiangsixingfenxi，jiangxiangsideguangpujuleichengfenzizu，xingchengyigefenziwangluo。wangluozhushichuanbo（NAP）weidangeguangputigongliaoguizhushihouxuan。shiyongClassyFireduizhexiehouxuanfenzijinxinghuaxuefenlei，ranhougenjumeigefenzijiazuzhongzuizhuyaodehuaxueleibieduifenzijiazujinxingjiadingzhushi。tongshi，liyongMS2LDAfenxiliaogongfashengpianduanhezhongxingsunshi（Mass2Motifs）defenbu，weiqitigongliaoyangpinjianyajiegouduoyangxinghefenbudexinxi。zhipuchengxiangzhipuchengxiang（MSI）shiyizhongjiyuzhipudefenzilizichengxiangjishu。zhipukeyicongbiaomianweimijidaxiaodequyuhuode，jianghuahewuzaibiaomian（weidianzixue，zuzhiqiepian）shangdehengxiangfenbuzhuanhuanweituxiang，jineryuguangxuetuxiangxiangguanlian。yixiechangjiandedianlijishubaokuoDESIchengxiang，MALDIchengxiangheercilizizhipuchengxiang（SIMSchengxiang）。MSInenggouduifuzabiaomiandeguangfanfenzijinxingwubiaojijiancehedingwei，tayichengweizhiyaoheyixueyanjiuzhongyigeyouxiyinlidefenzizuzhixuegongju。zi2005nianyilai，MSIzhujianyingyongyuzhiwuyanjiu(Imai et al., 2005；Mullen et al., 2005)。danbaizhihedaixiechanwudekongjianzuzhixinxijiangdadatigaowomenduizhiwudaixiehetedingzhiwuzuzhishenghuagongnengderenshi(Lee et al., 2012；Matros and Mock, 2013)。MSIshiyandehexinshizhipuyi，tayouliziyuan、zhipufenxiyihetanceqisandabufenzucheng。zailiziyuanzhong，fenxiwubeijiexihedianli；zaifenxiyizhong，tamengenjuzhiliangdianhebi（m/z）beifenli；zuihou，fenlidelizizaitanceqizhongbeijiancedao。zuoweizuizhongshuchu，tongguozaim/zkedushangxianshibeijiancelizideqiangdulaichanshengzhipu。weitanzhenMSIdejibenyuanlihenjiandan：yiqitongguoduizuzhiyangbendetedingquyujinxingguangzhahua，shoujiyixiliedezhipu。suihou，tongguohuizhifenxiwuzaizhipuzhongdedangem/zfengyux-yzuobiaodeqiangdu，shengchengfenxiwuzaiyangpinbiaomiandefenbutu(Goodwin et al., 2008；Svatos, 2010)。MALDIchengxiangshiyandedianxinggongzuoliuchengrutu4suoshi。tu4 dianxingdeMALDIchengxiangshiyanliucheng。yangpinchulishiMSIzhongzuiguanjiandebuzhou，yinweizhiyoushidangdeyangpinzhibeicainengbaochifenzidelaiyuan、fenbuhefengdu，quebaogaozhiliangdexinhaohezugoudekongjianfenbianlv(Grassl et al., 2011；Kaspar et al., 2011；Peukert et al., 2012；Spengler, 2014)。zhiwuzuzhideMSIyangpinzhibeifangfabiburudongwuzuzhidegengjutiaozhanxing(Boughton et al., 2015；Heyman and Dubery, 2016)。gaodengzhiwutidejiaozhicengdaibiaoliaoneibudaixiechanwu，shizhijiezhipuchengxiangdediyigezhangai，yinweidaduoshuruanhuajishu（ruMALDIheDESI）nanyichuantoutamen(Thunig et al., 2011)。zhiwuxibaobishixibaoneifenzizhipuchengxiangdedierpingzhang。liru，dangjizhirongyependaozhiwutibiaomianshi，xibaobihuizuzhirongyezaixibaobishangkuosan，daozhiMALDIchengxiangzhongfenxiwutiquxiaolvjiangdi(Takahashi et al., 2015)。zhiwuzuzhizhongdegaoshuifenhanliangzailengdongqiepianguochengzhongcunzailingyigetiaozhan。zhiwuzuzhizailengdongshigengweicuiruo，ciwai，duiyufuhanshuifendezhiwuyangben，yegengnanhuodewanzhengdebaoqiepian。zaituoshuiguochengzhongjingchangguanchadaoyangpinshousuohuobufenbaoluo，zhezhongxianxiangtongchanghuidaozhibiaomianbupingzheng，jineryingxiangMSIfenxi。xuyaozhuyideshi，yangpindeshousuoyekenengdaozhiMStuxiangheguangxuetuxiangzhijiandebupipei(Cha et al., 2008)，shishengwujieshibiandekunnan。xiamianshiguanyuzhipuchengxiangzaizhiwuyanjiuzhongdeyingyongjuli。tu5 jiaozhicengxiadaixiechanwudezhipuchengxiang。（i）ninanjieyepiandeMALDIchengxiang。yongniezijiazhuyedezuobian，zhongjianbuchuli，youbianjinlvfang（b）。shannaifen（a）heshannaifenshulitangzuo（d）zaiqianjiahelvfangjinpaoqurongyijiancedao，erzailvfangjinpaoquC26（c）heC30（f）dezhifangsuanbeixidiao。weijiandingdefenxiwum/z=210（d）zailvfangjinziquxianshichugaofengdu。（ii）damaiyebiaopim/z=276（b）、298（c）he300（d）dezuojidingzuozuodeDESItuxiang。yebeimianbiaopidaibeibaoluo（a）。（iii）yinjichengxiangyuzhijieDESIchengxiangdebijiao。（A）jusifuyixishangyeyindejianjieDESIchengxiang。（B）zhijieDESIchengxiang，yilvfang，jiachunheshui（1:1:0.4，v/v/v）zuoweipenwurongji。（a）baimaigenyepiandeguangxuetuxiang。（a1）zaijusifuyixishangdeyeyinjideguangxuetuxiang。（b-e）fenbieweim/z 104、286、298he300deDESItuxiang。zhexieshujufenbiezhenghezi(Cha et al., 2008)、(Li et al., 2011)、(Li et al., 2013)he(Bjarnholt et al., 2014)。jiyuzhiwudeMSIxianqianjizhongzaifangfalunfangmian(Matros and Mock, 2013)，erxianzaidejishuyijingchengshu，yincitayebeiyongyujiejueshengwuxuezhongyaodewenti(Lee et al., 2012)。liru，zaizhiwuhuanjingjiaohu(Shroff et al., 2008,2015；Klein et al., 2015；Ryffel et al., 2015；Soares et al., 2015；Tata et al., 2015)，xindehuahewujianding(Jaeger et al., 2013；Debois et al., 2014)hegongnengjiyinzuxue(Korte et al., 2012；Li et al., 2013)duyouyidingdeyingyong。dajiayouxingqukeyizijiquliaojieo！xiao jietongguoyishangjigefangmiandejieshao，xiaoyuanxiangxinniduizhipuyinggaiyouliaoyigedazhidiliaojie，duiyuwanquanbuliaojiezhipuderen，kanwanjintiandewenzhangkenenghuanhuicunzaisidongfeidongdeqingkuang，budongyemeiguanxi，zhiyaonizhidaozhipuyounaxiefangmiandeyingyong，bingqiezaiyihouzuoxiangguandeshiyanshinenggouxiangdaozhipu，xiaoyuanjiusuanmeibaixiezhepianwenzhangla！buguanzenyang，zhipuduzhishiyigeshiyangongju，shifuwuyukeyande，zuoweiyigekeyangongzuozheniyaozuodejiushiruheliyonghaota，rangtafahuigengdadejiazhio！References：Bantscheff M, Boesche M, Eberhard D, et al. Robust and sensitive iTRAQ quantification on an LTQ Orbitrap mass spectrometer[J]. Molecular & Cellular Proteomics, 2008, 7(9): 1702-1713.Boughton B A, Thinagaran D, Sarabia D, et al. Mass spectrometry imaging for plant biology: a review[J]. Phytochemistry Reviews, 2016, 15(3): 445-488.Cha S, Zhang H, Ilarslan H I, et al. Direct profiling and imaging of plant metabolites in intact tissues by using colloidal graphite‐assisted laser desorption ionization mass spectrometry[J]. The Plant Journal, 2008, 55(2): 348-360.Debois D, Jourdan E, Smargiasso N, et al. Spatiotemporal monitoring of the antibiome secreted by Bacillus biofilms on plant roots using MALDI mass spectrometry imaging[J]. Analytical chemistry, 2014, 86(9): 4431-4438.Grassl J, Taylor N L, Millar A H. Matrix-assisted laser desorption/ionisation mass spectrometry imaging and its development for plant protein imaging[J]. Plant methods, 2011, 7(1): 1-11.Gygi S P, Rist B, Gerber S A, et al. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags[J]. Nature biotechnology, 1999, 17(10): 994-999.Heyman H M, Dubery I A. The potential of mass spectrometry imaging in plant metabolomics: a review[J]. Phytochemistry reviews, 2016, 15(2): 297-316.Imai T, Tanabe K, Kato T, et al. Localization of ferruginol, a diterpene phenol, in Cryptomeria japonica heartwood by time-of-flight secondary ion mass spectrometry[J]. Planta, 2005, 221(4): 549-556.Jaeger R J R, Lamsho?ft M, Gottfried S, et al. HR-MALDI-MS imaging assisted screening of β-carboline alkaloids discovered from Mycena metata[J]. Journal of Natural Products, 2013, 76(2): 127-134.Kang K B, Ernst M, van der Hooft J J J, et al. Comprehensive mass spectrometry‐guided phenotyping of plant specialized metabolites reveals metabolic diversity in the cosmopolitan plant family Rhamnaceae[J]. The Plant Journal, 2019, 98(6): 1134-1144.Kaspar S, Peukert M, Svatos A, et al. MALDI‐imaging mass spectrometry–an emerging technique in plant biology[J]. Proteomics, 2011, 11(9): 1840-1850.Klein A T, Yagnik G B, Hohenstein J D, et al. Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging[J]. Analytical chemistry, 2015, 87(10): 5294-5301.Korte A R, Song Z, Nikolau B J, et al. Mass spectrometric imaging as a high-spatial resolution tool for functional genomics: Tissue-specific gene expression of TT7 inferred from heterogeneous distribution of metabolites in Arabidopsis flowers[J]. Analytical Methods, 2012, 4(2): 474-481.Lee Y J, Perdian D C, Song Z, et al. Use of mass spectrometry for imaging metabolites in plants[J]. The Plant Journal, 2012, 70(1): 81-95.Li B, Bjarnholt N, Hansen S H, et al. Characterization of barley leaf tissue using direct and indirect desorption electrospray ionization imaging mass spectrometry[J]. Journal of mass spectrometry, 2011, 46(12): 1241-1246.Li B, Knudsen C, Hansen N K, et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging[J]. The Plant Journal, 2013, 74(6): 1059-1071.Matros A, Mock H P. Mass spectrometry based imaging techniques for spatially resolved analysis of molecules[J]. Frontiers in Plant Science, 2013, 4: 89.Mergner J, Frejno M, List M, et al. Mass-spectrometry-based draft of the Arabidopsis proteome[J]. Nature, 2020, 579(7799): 409-414.Mullen A K, Clench M R, Crosland S, et al. Determination of agrochemical compounds in soya plants by imaging matrix‐assisted laser desorption/ionisation mass spectrometry[J]. Rapid Communications in Mass Spectrometry: An International Journal Devoted to the Rapid Dissemination of Up‐to‐the‐Minute Research in Mass Spectrometry, 2005, 19(18): 2507-2516.Oksman-Caldentey K M, Saito K. Integrating genomics and metabolomics for engineering plant metabolic pathways[J]. Current opinion in biotechnology, 2005, 16(2): 174-179.Ong S E, Kratchmarova I, Mann M. Properties of 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC)[J]. Journal of proteome research, 2003, 2(2): 173-181.Peukert M, Matros A, Lattanzio G, et al. Spatially resolved analysis of small molecules by matrix‐assisted laser desorption/ionization mass spectrometric imaging (MALDI‐MSI)[J]. New Phytologist, 2012, 193(3): 806-815.Piques M, Schulze W X, H?hne M, et al. Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis[J]. Molecular systems biology, 2009, 5(1): 314.Ryffel F, Helfrich E J N, Kiefer P, et al. Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves[J]. The ISME journal, 2016, 10(3): 632-643.Shroff R, Schramm K, Jeschke V, et al. Quantification of plant surface metabolites by MALDI mass spectrometry imaging: glucosinolates on Arabidopsis thaliana leaves[J]. Plant J, 2015, 81: 961-972.Shroff R, Vergara F, Muck A, et al. Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense[J]. Proceedings of the National Academy of Sciences, 2008, 105(16): 6196-6201.Soares M S, da Silva D F, Forim M R, et al. Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry[J]. Phytochemistry, 2015, 115: 161-170.Spengler B. Mass spectrometry imaging of biomolecular information[J]. Analytical chemistry, 2015, 87(1): 64-82.Takahashi K, Kozuka T, Anegawa A, et al. Development and application of a high-resolution imaging mass spectrometer for the study of plant tissues[J]. Plant and Cell Physiology, 2015, 56(7): 1329-1338.Tata A, Perez C J, Ore M O, et al. Evaluation of imprint DESI-MS substrates for the analysis of fungal metabolites[J]. RSC advances, 2015, 5(92): 75458-75464.Thunig J, Hansen S H, Janfelt C. Analysis of secondary plant metabolites by indirect desorption electrospray ionization imaging mass spectrometry[J]. Analytical chemistry, 2011, 83(9): 3256-3259.Venable J D, Wohlschlegel J, McClatchy D B, et al. Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer[J]. Analytical chemistry, 2007, 79(8): 3056-3064.Wolfender J L, Rudaz S, Hae Choi Y, et al. Plant metabolomics: from holistic data to relevant biomarkers[J]. Current Medicinal Chemistry, 2013, 20(8): 1056-1090.Zhao C, Zayed O, Yu Z, et al. Leucine-rich repeat extensin proteins regulate plant salt tolerance in Arabidopsis[J]. Proceedings of the National Academy of Sciences, 2018, 115(51): 13123-13128.NO.1haowentuijianHistorical articleszhuanluyinziyanjiutaolu（er）danbaifanyihouxiushi——linsuanhua（yi）【zhizhao】jiaomushaikucunzaizijihuozenmeban？【xiaoyuandayi】naxiekunraonideCo-IPjiancewentibianqingdiwo，tongyizhanxian——quedingjiyinshangxiayouguanxi【xiaoyuandayi】Co-IPyijiIP-MSxiangguanshiyanwentijiehuoNO.2womennengtigongdefuwuTechnical servicezhipuIP-MS
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